Response of Chick's Comb to Naturally Occurring Androgens and Estrogens *

نویسندگان

  • Ralph I. Dorfman
  • William Walter Greulich
چکیده

The fact that the growth of the chick's comb is accelerated by the administration of androgenic material has been demonstrated by Ruzicka,3 by Burrows, Byerly, and Evans,' and by Frank and Klempner.' Ruzicka painted the chick's comb with a 0.5 per cent solution of androsterone in oil each day for a period of several weeks and obtained large increases in comb area. He did not, however, study this reaction quantitatively. Frank and Klempner applied the androgens in oil solutions directly to the base of the comb of white leghorn chicks. Applications were begun on the sixth day after hatching and were repeated on ten successive days. The animals were sacrificed and the comb weights were determined on the day following the last application. These workers were able to evoke a definite response with as little as 20 micrograms of androsterone. Burrows and his co-workers injected both androsterone and testosterone either into the base of the chick's comb or into the breast muscles and found that both of these androgens stimulated comb growth. We have investigated the response of the chick's comb to androgens, with a view to its possible utilization in a quantitative method of assaying androgenic activity. We have attempted, therefore, to determine the sensitivity, the specificity, and the reproducibility of this biological reaction. Approximately two thousand male and female single-combed, white leghorn chicks from a carefully selected, homogeneous stock were used in this study. All the animals were fed the same adequate diet. The hormones employed were dissolved in olive oil and the various quantities injected were always

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عنوان ژورنال:
  • The Yale Journal of Biology and Medicine

دوره 10  شماره 

صفحات  -

تاریخ انتشار 1937